Writer-performer Charles Busch will be heading back to downtown Gotham’s Theater for the New City to preem his latest play, “Judith of Bethulia.”

Scribe’s most recent outing, “The Divine Sister,” also bowed at TNC in a 2010 run that yielded a commercial transfer to the Soho Playhouse, where the comedy carved out a 7 1/2-month run.

New show, a spoof of Hollywood biblical epics, is inspired by the 1914 silent pic of the same name about a Jewish widow who poses as a concubine to infiltrate an army of invading Assyrians. Busch, who played the mother superior in “Divine Sister,” will play the title role in “Judith.”

Frequent Busch collaborator Carl Andress, helmer of wimple comedy “Divine Sister,” directs. Along with topliner Busch, cast of “Judith” includes Mary Testa, Dave August, Larry Bullock, Kendal Sparks, Jennifer Van Dyck and Billy Wheelan.

Limited engagement of “Judith” runs March 30-April 28.