The Roundabout Theater Company has reassembled the cast of its Tony-winning revival of “Assassins,” including Neil Patrick Harris, for a December benefit performance of the musical.

Michael Cerveris, who picked up a 2004 Tony for his perf as John Wilkes Booth in the show, also is among the returning thesps, as are Denis O’Hare, Becky Ann Baker and Mario Cantone, among others. Director Joe Mantello, who scored a Tony for his own work on the production, returns to helm.

The benefit concert reading, which will raise funds for the Gotham nonprofit, is the latest mark of the Roundabout’s ongoing relationship with composer Stephen Sondheim, who penned 1991 tuner “Assassins” with book writer John Weidman. The Roundabout named its third Broadway space in honor of Sondheim, after it had produced a string of productions of the composer’s work including “Company,” “Sunday in the Park with George” and “Pacific Overtures.”

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One-night-only “Assassins” event is set for Dec. 3 at Studio 54, also the home of the 2004 staging.